Cell Signaling Technology

Product Pathways - Adhesion

Integrin α6 Antibody #3750

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 150, 125 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin α6 Antibody detects endogenous levels of total integrin α6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Gly571 of human integrin α6. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using Integrin α6 Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).Integrin α6 is a 120 kDa protein with two splice variants, integrin α6, 6A and 6B (3), which function as receptors for laminins on the basal membrane to mediate cellular adhesion events (4-6). α6 integrins have been shown to play an important role in hematopoietic stem and progenitor cell homing to the bone marrow.

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. Hogervorst, F. et al. (1993) J Cell Biol 121, 179-91.
  4. Sonnenberg, A. et al. (1990) J Cell Biol 110, 2145-55.
  5. Sonnenberg, A. et al. (1993) J Cell Sci 106 ( Pt 4), 1083-102.
  6. Shaw, L.M. et al. (1990) J Cell Biol 110, 2167-74.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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