Product Pathways - Phosphatases
SHP-2 Antibody #3752
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R | 72 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
SHP-2 Antibody detects endogenous levels of total SHP-2 independent of phosphorylation.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the amino-terminal domain of human SHP-2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from C6, NIH/3T3, 293 and HeLa cells, using SHP-2 Antibody.
Background
SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (4).
- Qu, C.K. (2000) Cell Res 10, 279-88.
- Maroun, C. R. et al. (2000) Mol Cell Biol 20, 8513-25.
- Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72.
- Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90.
Application References
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