Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

JunB (C37F9) Rabbit mAb #3753

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC ChIP H M R Mk Endogenous 42, 43 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

JunB (C37F9) Rabbit mAb detects endogenous levels of total JunB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro169 of human JunB.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using JunB (C37F9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JunB (C37F9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using JunB (C37F9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of JunB (C37F9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).

  1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
  2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
  3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
  4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
  5. Chiu, R. et al. (1989) Cell 59, 979-986.
  6. Schütte, J. et al. (1989) Cell 59, 987-997.
  7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
  8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
  9. Passegué, E. et al. (2004) Cell 119, 431-443.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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