Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

JunB (P169) Antibody #3755

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M (R) Endogenous 42, 43 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

JunB (P169) Antibody detects the endogenous level of total JunB protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro169 of human JunB. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from various cell lines using JunB (P169) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using JunB (P169) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).

  1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
  2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
  3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
  4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
  5. Chiu, R. et al. (1989) Cell 59, 979-986.
  6. Schütte, J. et al. (1989) Cell 59, 987-997.
  7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
  8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
  9. Passegué, E. et al. (2004) Cell 119, 431-443.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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