Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 555 Conjugate) #3756
|3756S||100 µl (50 assays)||---||In Stock||---|
|3756||carrier free and custom formulation / quantity||email request|
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|IF-IC||1:50||Transfected Only||Mouse IgG2a|
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 555 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescence in cells transfected with Myc-tagged protein.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.