Cell Signaling Technology

Product Pathways -

Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 555 Conjugate) #3756

Applications Reactivity Sensitivity Isotype
IF-IC All Transfected Only Mouse IgG2a

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 555 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.

IF-IC

IF-IC

Confocal immunofluorescent analysis of COS cells transiently transfected with a Myc-tagged fusion protein using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 555 Conjugate) (red) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3623 (green).

Description

This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescence in cells transfected with Myc-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

  1. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products