Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

SNIP/p140Cap Antibody #3757

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 145 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SNIP/p140Cap Antibody detects endogenous levels of total SNIP/p140Cap protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SNIP/p140Cap. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562, SH-SY5Y, IMR32, Neuro2A and PC12 cell lines using SNIP/p140Cap Antibody.

Background

SNIP (SNAP25-interacting protein)/p140Cap (p130Cas-associated protein) is a cytoskeleton-associated protein identified initially in rat as a protein interacting with the brain-specific synaptosome protein SNAP25 (1) and subsequently as interacting with the broadly expressed scaffold protein p130Cas (2). SNAP25, a presynaptic protein implicated in neurotransmitter secretion, membrane fusion and neurite outgrowth, is part of the SNARE complex that includes syntaxin and synaptobrevin/VAMP (3). SNIP-SNAP25 association is mediated by coiled-coil interactions (1). Overexpression of SNIP inhibits calcium-dependent exocytosis in PC12 cells (1). Human and mouse orthologs of SNIP, termed p140Cap, were subsequently identified through association with p130Cas, a substrate of v-Src and v-Crk that is tyrosine-phosphorylated in response to cell adhesion and mitogenic stimuli (2,4,5). Expression of p140Cap was observed in brain, testis and epithelial-rich tissues and may exist in various alternatively spliced, tissue-specific isoforms (2). p140Cap is also tyrosine-phosphoryalated in response to adhesion molecules and EGF treatment (2). Together these studies suggest a role for SNIP/p140Cap in controlling cell spreading, migration and neurosecretion.

  1. Chin, L.S. et al. (2000) J. Biol. Chem. 275, 1191-1200.
  2. Di Stefano, P. et al. (2004) Mol. Biol. Cell 15, 787-800.
  3. Gerst, J.E. (1999) Cell. Mol. Life Sci. 55, 707-734.
  4. Sakai, R. et al. (1994) EMBO J. 13, 3748-3756.
  5. Defilippi, P. et al. (2006) Trends Cell Biol. 16, 257-263.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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