Product Pathways - Phosphatases
SHP-1 (C14H6) Rabbit mAb #3759
PhosphoSitePlus® protein, site, and accession data: SHP-1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M (R) | Endogenous | 68 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
SHP-1 (C14H6) Rabbit mAb detects endogenous level of total SHP-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro161 of human SHP-1.
Western Blotting
Western blot analysis of extracts from Jurkat, 32D and BaF3 cells using SHP-1 (C14H6) Rabbit mAb.
Background
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6).
- Yi, T.L. et al. (1992) Mol Cell Biol 12, 836-46.
- Yi, T. et al. (1995) Blood 85, 87-95.
- Keilhack, H. et al. (2001) J Cell Biol 152, 325-34.
- Yi, T. et al. (1993) Mol Cell Biol 13, 7577-86.
- Wu, C. et al. (2003) Gene 306, 1-12.
- Bhattacharya, R. et al. (2008) J Mol Signal 3, 8.
Application References
- Blaser, C. et al. (2011) PLoS One 6, e17840. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
