Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-IP3 Receptor (Ser1756) Antibody #3760

Applications Reactivity MW (kDa) Source
W H M R 320 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-IP3 Receptor (Ser1756) Antibody detects endogenous levels of IP3 Receptor only when phosphorylated at Ser1756.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser1756 of rat IP3 receptor. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain using Phospho-IP3 Receptor (Ser1756) Antibody. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with IP3 Receptor (Ser1756) phosphopeptide or with IP3 Receptor (Ser1756) non-phosphopeptide prior to incubating the membrane.

Background

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family, and is located in the endoplasmic reticulum. It functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3 receptor 1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual receptor subunits, resulting in homo- or hetero-oligomerization of the receptor isoform, form a functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release (3,4). IP3R1 mediated calcium release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).

  1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
  2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
  3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
  4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
  5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

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