Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

IP3 Receptor Antibody #3763

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 320 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IP3 Receptor Antibody detects endogenous levels of total IP3 receptor type 1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Met2750 of human IP3 receptor. Antibodies are purified by protein A chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using IP3 Receptor Antibody.

Background

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. IP3R functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2, and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual subunits homo- or hetero-oligomerize to form the receptor's functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may be a mode of regulation for Ca2+ release (3,4). IP3R1-mediated Ca2+ release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).

  1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
  2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
  3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
  4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
  5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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