Product Pathways - Apoptosis
BNIP3 Antibody (Rodent Specific) #3769
|3769S||100 µl (10 western blots)||---||In Stock||---|
|3769||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Mouse, Rat||Endogenous||22-28, 50-55||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
BNIP3 Antibody (Rodent Specific) detects endogenous levels of total BNIP3 protein from mouse and rat.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser60 of BNIP3 from mouse/rat. Antibodies were purified by peptide affinity chromatography.
Western blot analysis of extracts from C2C12 cells, untreated (-) or cobalt chloride-treated (100 μM; overnight) using BNIP3 Antibody (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
BNIP3 (Bcl-2/E1B-19kDa interacting protein 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain and a carboxyl terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kDa, it runs anomalously on SDS-PAGE and includes a band of around 60 kDa that may be a dimeric form that is not reduced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-19kDa. BNIP3 is distinct from other Bcl-2 family members that contain only the BH3 domain in that the TM domain, and not the BH3 domain, is required for mitochondrial targeting and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6,7), although the relationship between BNIP3 and these processes is not fully understood. Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1α (8-10). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (11-14).
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- Shackelford, D.B. et al. (2009) Proc Natl Acad Sci U S A 106, 11137-42. Applications: Western Blotting.
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