Product Pathways - Jak/Stat Pathway
Phospho-Jak2 (Tyr221) Antibody #3774
|3774S||100 µl (10 western blots)||---||In Stock||---|
|3774||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Rat.
Specificity / Sensitivity
Phospho-Jak2 (Tyr221) Antibody detects endogenous levels of Jak2 only when phosphorylated at Tyr221.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr221 of human Jak2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
Jak2 is autophosphorylated at Tyr1007/1008 in the putative activation loop during cytokine signaling (6). Tyr221 and 570 have also been shown to be prominent sites for autophosphorylation (7,8). Mutational analysis suggests that phosphorylation at Tyr221 may increase kinase activity, while phosphorylation at Tyr570, which lies within the JH2 inhibitory domain, may contribute to inhibiting Jak2 activity. In addition, Tyr813 was identified as a site for autophosphorylation critical for the activation of Jak2 by the SH2 domain-containing protein SH2-B β (9).
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
- Gauzzi, M.C. et al. (1996) J Biol Chem 271, 20494-500.
- Argetsinger, L.S. et al. (2004) Mol Cell Biol 24, 4955-67.
- Feener, E.P. et al. (2004) Mol Cell Biol 24, 4968-78.
- Kurzer, J.H. et al. (2004) Mol Cell Biol 24, 4557-70.
- Okumura, M. et al. (2011) Biochem Pharmacol , . Applications: Western Blotting.
- Pulikkan, J.A. et al. (2012) Blood , . Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.