Product Pathways - Jak/Stat Pathway
Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M B (R) (Mk) | Endogenous | 125 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb detects endogenous levels of Jak2 when phosphorylated at Tyr1007/1008. This antibody can also detect single phosphorylation at either 1007 or 1008. Cross-reactivity may be observed with phosphorylated Tyk2.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to a region surrounding Tyr1007/1008 of human Jak2.
Western Blotting
Westen blot analysis of extracts from BaF3 cells overexpressing murine Jak2 and thrombopoietin (TPO) receptor, untreated or TPO-treated, using Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb (top) or total Jak2 (24B11) Rabbit mAb #3229 (bottom). Cell line was kindly provided by Dr. Stefan Constantinescu, Ludwig Institute for Cancer Research, Brussels, Belgium.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-1635.
- VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
- Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
- Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
