Cell Signaling Technology

Product Pathways - MAPK Signaling

GCK Antibody #3782

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 91 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GCK Antibody detects endogenous levels of total GCK protein. This antibody does not cross-react with HPK or other members of the GCK family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser380 of human GCK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using GCK Antibody.

Background

Germinal center kinase (GCK) is the founding member of the GCK family, a group of serine/threonine kinases homologous to the yeast Ste20 kinase. GCK is activated by TNF and associated with TRAF2 (TNF receptor-associated factor 2) and MEKK1, thereby activating the SAPK/JNK pathway (1,2). GCK does not significantly phosphorylate MEKK1, instead it enhances MEKK1 oligomerization and autophosphorylation (3). GCK binds and activates MLK3, indicating that GCK may have other effectors (3).

  1. Pombo, C. M. et al. (1995) Nature 377, 750-754.
  2. Yuasa, T. et al. (1998) J. Biol. Chem. 273, 22681-22692.
  3. Chadee, D. N. et al. (2002) Mol. Cell. Biol. 22, 737-749.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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