Product Pathways - MAPK Signaling
GCK Antibody #3782
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk | Endogenous | 91 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
GCK Antibody detects endogenous levels of total GCK protein. This antibody does not cross-react with HPK or other members of the GCK family.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding Ser380 of human GCK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using GCK Antibody.
IF-IC
Immunocytochemical staining of NIH/3T3 cells, using GCK Antibody in the presence (left) or absence (right) of GCK blocking peptide.
Background
Germinal center kinase (GCK) is the founding member of the GCK family, a group of serine/threonine kinases homologous to the yeast Ste20 kinase. GCK is activated by TNF and associated with TRAF2 (TNF receptor-associated factor 2) and MEKK1, thereby activating the SAPK/JNK pathway (1,2). GCK does not significantly phosphorylate MEKK1, instead it enhances MEKK1 oligomerization and autophosphorylation (3). GCK binds and activates MLK3, indicating that GCK may have other effectors (3).
- Pombo, C. M. et al. (1995) Nature 377, 750-754.
- Yuasa, T. et al. (1998) J. Biol. Chem. 273, 22681-22692.
- Chadee, D. N. et al. (2002) Mol. Cell. Biol. 22, 737-749.
Application References
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
