Product Pathways - Tyrosine Kinase / Adaptors
Phospho-HER4/ErbB4 (Tyr984) Antibody #3790
PhosphoSitePlus® protein, site, and accession data: HER4
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 180 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3790:
- Western Blotting
Specificity / Sensitivity
Phospho-HER4/ErbB4 (Tyr984) Antibody detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr984. This antibody may cross-react with other tyrosine-phosphorylated proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr984 of human HER4/ErbB4. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from T-47D cells, untreated or heregulin-stimulated, using Phospho-HER4/ErbB4 (Tyr984) Antibody (upper) and HER4/ErbB4 (111B2) Rabbit mAb#4795 (lower).
Background
The HER/ErbB receptor tyrosine kinase family has been implicated in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).
- Holbro, T. and Hynes, N.E. (2004) Annu Rev Pharmacol Toxicol 44, 195-217.
- Roskoski, R. (2004) Biochem Biophys Res Commun 319, 1-11.
- Carpenter, G. (2003) Exp Cell Res 284, 66-77.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4757 Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb
- 4795 HER4/ErbB4 (111B2) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
