Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CIITA Antibody #3793

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Transfected Only 140 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CIITA Antibody detects transfected levels of total CIITA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu149 of human CIITA. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, mock transfected or transfected with human CIITA, using CIITA Antibody.

Background

MHC class II (MHC-II) proteins play critical roles in cellular immune responses and their expression is mainly regulated by the non-DNA binding transcription factor CIITA (MHC class II transactivator) (1,2). CIITA expression is upregulated by IFN-γ and it in turn enchances MHC-II expression and represses collagen expression (3,4). CIITA has a limited number of transcriptional targets, most of which are involved in MHC-mediated antigen presentation (5). Mutations in the CIITA are associated with the hereditary immunodeficiency disease Bare Lymphocyte Syndrome (BLS) which is characterized by a nearly complete absence of MHC-II expression (also referred to as MHC-II deficiency) (6,7).

  1. Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
  2. Drozina, G. et al. (2005) Curr Top Microbiol Immunol 290, 147-70.
  3. Dong, Y. et al. (1999) J Immunol 162, 4731-9.
  4. Butticè, G. et al. (2006) Circ Res 98, 472-9.
  5. Krawczyk, M. et al. (2008) PLoS Genet 4, e1000058.
  6. Reith, W. and Mach, B. (2001) Annu Rev Immunol 19, 331-73.
  7. Waldburger, J.M. et al. (2000) Immunol Rev 178, 148-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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