Product Pathways - Development
Frizzled5 Antibody #3795
|W IP||H||Transfected Only||50-65||Rabbit|
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Frizzled5 Antibody detects transfected levels of total frizzled5 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to the amino acid sequence surrounding Ala549 of human frizzled5. Antibodies are purified by protein A and peptide affinity chromatography.
Frizzled (Fzd) belongs to the seven transmembrane-spanning G-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca2+ pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11).
- Schulte, G. and Bryja, V. (2007) Trends Pharmacol Sci 28, 518-25.
- Hsieh, J.C. et al. (1999) Proc Natl Acad Sci USA 96, 3546-51.
- Wong, H.C. et al. (2003) Mol Cell 12, 1251-60.
- Zeng, X. et al. (2005) Nature 438, 873-7.
- Davidson, G. et al. (2005) Nature 438, 867-72.
- Fanto, M. and McNeill, H. (2004) J Cell Sci 117, 527-33.
- Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46.
- Cadigan, K.M. and Liu, Y.I. (2006) J Cell Sci 119, 395-402.
- Xu, Q. et al. (2004) Cell 116, 883-95.
- Nam, J.S. et al. (2006) J Biol Chem 281, 13247-57.
- Hendrickx, M. and Leyns, L. (2008) Dev Growth Differ 50, 229-43.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2721 Wnt3a (C64F2) Rabbit mAb
- 2530 Wnt5a/b (C27E8) Rabbit mAb
- 2560 LRP6 (C5C7) Rabbit mAb
- 2568 Phospho-LRP6 (Ser1490) Antibody
- 3224 Dvl2 (30D2) Rabbit mAb
- 2201 Naked1 (C30F10) Rabbit mAb
- 2596 Naked2 (C36D3) Rabbit mAb
- 2504 APC Antibody
- 2087 Axin1 (C76H11) Rabbit mAb
- 2074 Axin1 (C95H11) Rabbit mAb
- 2151 Axin2 (76G6) Rabbit mAb
- 2230 LEF1 (C12A5) Rabbit mAb
- 2203 TCF1 (C63D9) Rabbit mAb
- 2569 TCF4 (C48H11) Rabbit mAb
- 9323 Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb
- 9315 GSK-3β (27C10) Rabbit mAb
- 9582 β-Catenin (6B3) Rabbit mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.