Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Metabolism

α-Amylase (D55H10) XP® Rabbit mAb #3796

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H R (M) Endogenous 58 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

α-Amylase (D55H10) XP® Rabbit mAb detects endogenous levels of total α-amylase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α-amylase.

Western Blotting

Western Blotting

Western blot analysis of extracts from AR42J cells using α-Amylase (D55H10) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human pancreas using α-Amylase (D55H10) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat pancreas using α-Amylase (D55H10) XP® Rabbit mAb.


Background

α-amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). Studies reported the release of an ectopically expressed α-amylase in certain tumors (1). Furthermore, a new type of α-amylase (carcinoid α-amylase or AMY2B) was identified in a lung carcinoid tissue (2-4). The ectopic expression of α-amylase in a neuroendocrine tumor was also reported (5).

  1. Tomita, N. et al. (1988) Cancer Res 48, 3292-6.
  2. Tomita, N. et al. (1989) Gene 76, 11-8.
  3. Groot, P.C. et al. (1989) Gene 85, 567-8.
  4. Yokouchi, H. et al. (1990) Gene 90, 281-6.
  5. Daa, T. et al. (2002) J Exp Clin Cancer Res 21, 131-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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