Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

ERp44 (D17A6) XP® Rabbit mAb #3798

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Mk Endogenous 44 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ERp44 (D17A6) XP® Rabbit mAb detects endogenous levels of total ERp44 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ERp44.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using ERp44 (D17A6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERp44 (D17A6) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Background

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum resident protein 44 (ERp44), constitute the PDI family (2). ERp44 is induced upon ER stress and is linked to Ero1-Lα and Ero1-Lβ through mixed disulfide bonds (3). ERp44 was shown to mediate the ER localization of Ero1-Lα (4).

  1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
  2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
  3. Anelli, T. et al. (2002) EMBO J 21, 835-44.
  4. Anelli, T. et al. (2003) EMBO J 22, 5015-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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