Product Pathways - Translational Control
PPIG Antibody #3803
|W||H M R||Endogenous||110||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
PPIG Antibody detects endogenous levels of total PPIG protein. This antibody also recognizes a unidentified protein at 49 kDa.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu391 of human PPIG. Antibodies are purified by peptide affinity chromatography.
PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3,4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7,8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9).
- Fischer, G. et al. (1989) Nature 337, 476-8.
- Freskgård, P.O. et al. (1992) Science 258, 466-8.
- Nestel, F.P. et al. (1996) Gene 180, 151-5.
- Mortillaro, M.J. and Berezney, R. (1998) J Biol Chem 273, 8183-92.
- Meier, U.T. and Blobel, G. (1992) Cell 70, 127-38.
- Zahler, A.M. et al. (1993) Science 260, 219-22.
- Lin, C.L. et al. (2004) Biochem Biophys Res Commun 321, 638-47.
- Bourquin, J.P. et al. (1997) Nucleic Acids Res 25, 2055-61.
- Dubourg, B. et al. (2004) J Biol Chem 279, 22322-30.
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For Research Use Only. Not For Use In Diagnostic Procedures.