Cell Signaling Technology

Product Pathways - Translational Control

Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb #3806

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Mk Endogenous 200 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb detects endogenous levels of rictor protein only when phosphorylated at Thr1135.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1135 of human rictor protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved HeLa cells, untreated or treated with IGF-1 (50 ng/ml, 30 minutes) and λ phosphatase, using Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb (upper) or Rictor (53A2) Rabbit mAb #2114 (lower).

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity (6-8).

  1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
  2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
  4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.
  5. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  6. Dibble, C.C. et al. (2009) Mol Cell Biol 29, 5657-70.
  7. Julien, L.A. et al. (2010) Mol Cell Biol 30, 908-21.
  8. Treins, C. et al. (2010) Oncogene 29, 1003-16.

Application References

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Companion Products

This product and its use may be covered by one or more patent applications owned by Cell Signaling Technology, Inc. including US Patent Publication No. 2009/0203043.

For Research Use Only. Not For Use In Diagnostic Procedures.

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