Cell Signaling Technology

Product Pathways - Metabolism

Enolase-1 Antibody #3810

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 47 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Enolase-1 Antibody detects endogenous levels of total enolase-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Enolase-1 Antibody.

Background

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner (1). Enolase-1 plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion (2,3). Abnormal expression of enolase-1 is associated with tumor progression in some cases of breast and lung cancer (4-7). Alternatively, an enolase-1 splice variant (MBP-1) binds the c-myc promoter p2 and may function as a tumor suppressor. For this reason enolase-1 is considered as a potential therapeutic target in the treatment of some forms of cancer (8).

  1. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
  2. Redlitz, A. et al. (1995) Eur J Biochem 227, 407-15.
  3. Jiang, B.H. et al. (1997) Cancer Res 57, 5328-35.
  4. Peebles, K.A. et al. (2003) Carcinogenesis 24, 651-7.
  5. Zhang, L. et al. (2000) J Surg Res 93, 108-19.
  6. Wu, W. et al. (2002) Clin Exp Metastasis 19, 319-26.
  7. Hennipman, A. et al. (1988) Tumour Biol 9, 241-8.
  8. Feo, S. et al. (2000) FEBS Lett 473, 47-52.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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