Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PI3 Kinase Class III Antibody #3811

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 100 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PI3 Kinase Class III Antibody detects endogenous levels of total PI3K class III protein. This antibody may cross-react with an unidentified protein at ~55 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human PI3K class III. Antibodies are purified peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 and RN33B cells using PI3 Kinase Class III Antibody.

Background

Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5).

  1. Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229-35.
  2. Panaretou, C. et al. (1997) J Biol Chem 272, 2477-85.
  3. Kihara, A. et al. (2001) J Cell Biol 152, 519-30.
  4. Corvera, S. (2001) Traffic 2, 859-66.
  5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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