Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

LSP1 Antibody #3812

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 52 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LSP1 Antibody detects endogenous levels of LSP1 protein independent of phosphorylation.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 37-48 of human LSP1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human B cell lines Daudi and Ramos (lanes 1 and 2), from mouse spleenocyte (lane 3), and from the B cell line A20 (lane 4), using LSP1 Antibody.

Background

LSP1 is a 52 kDa leukocyte-specific protein expressed in B cells, T cells, macrophages and neutrophils (1). The protein binds F-actin through its C-terminal actin-binding repeat and regulates cytoskeletal-related cellular movement such as polarization, chemotaxis and locomotion (2-4). LSP1 is a phospho-protein and has been identified as a major substrate of MAP kinase and PKC in lymphocytes and neutrophils (5-7). The phosphorylation is possibly related to its cytoskeletal regulatory function.

  1. Li, Y. et al. (1995) J. Immunol. 155, 3563-3569.
  2. Zhang, Q. et al. (2000) J. Immunol. 165, 2052-2058.
  3. Li, Y. et al. (2000) Blood 96, 1100-1105.
  4. Wang, C. et al. (2002) J. Immunol. 169, 415-423.
  5. Huang, C. K. et al. (1997) J. Biol. Chem. 272, 17-19.
  6. Matsumoto, N. et al. (1995) J. Biochem. (Tokyo) 117, 222-229.
  7. Carballo, E. et al. (1996) J. Immunol. 156, 1709-1713.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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