Product Pathways - Neuroscience
Plexin A1 Antibody #3813
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP IHC-P | H R (M) | 211 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Plexin A1 Antibody detects endogenous levels of total Plexin A1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) surrounding Gln1875 of human Plexin A1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from fetal rat brain and K562 cells using Plexin A1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Plexin A1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using Plexin A1 Antibody in the presence of control peptide (left) or antigen specific peptide (right).
Background
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).
- Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
- Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
- Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
Application References
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
