Cell Signaling Technology

Product Pathways - Apoptosis

Bak Antibody #3814

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Bak Antibody detects endogenous levels of Bak protein. No cross reactivity was detected with other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly82 of human Bak. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from OVCAR8, HT29 and Jurkat cells using Bak Antibody.

Background

Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).

  1. Gross, A. et al. (1999) Genes Dev. 13, 1899-1911.
  2. Wei, M.C. et al. (2001) Science 292, 727-730.
  3. Zong, W.X. et al. (2001) Genes Dev. 15, 1481-1486.
  4. Degenhardt, K. et al. (2002) J. Biol. Chem. 277, 14127-14134.
  5. Wei, M.C. et al. (2000) Genes Dev. 14, 2060-2071.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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