Cell Signaling Technology

Product Pathways - Glucose Metabolism

GFAT1 Antibody #3818

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 80 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

GFAT1 Antibody detects endogenous levels of total GFAT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GFAT1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GFAT1 Antibody.

Background

GFAT1, glutamine:fructose-6-phosphate aminotransferase 1, is the rate-limiting enzyme of the hexosamine biosynthesis pathway (1). This enzyme catalyses the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate and glutamate (2). The hexosamine biosynthesis pathway generates the building blocks for protein and lipid glycosylation (2). Furthermore, studies suggest that increased activity of this pathway is a contributing factor to hyperglycemia-induced insulin resistance (1,2). GFAT1 is more active in non-insulin-dependent diabetes mellitus (NIDDM) patients (3). Transgenice mice overexpressing this enzyme in skeletal muscle and adipose tissue show an insulin resistance phenotype (4,5).

  1. Niimi, M. et al. (2001) J Hum Genet 46, 566-71.
  2. DeHaven, J.E. et al. (2001) Diabetes 50, 2419-24.
  3. Yki-Järvinen, H. et al. (1999) Life Sci 65, 215-23.
  4. Cooksey, R.C. et al. (1999) Endocrinology 140, 1151-7.
  5. Hebert, L.F. et al. (1996) J Clin Invest 98, 930-6.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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