Cell Signaling Technology

Product Pathways - Metabolism

PDHK1 (C47H1) Rabbit mAb #3820

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk (B) Endogenous 47 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PDHK1 (C47H1) Rabbit mAb detects endogenous levels of total PDHK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDHK1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PDHK1 (C47H1) Rabbit mAb.

Background

Pyruvate generated from glycolysis is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) under normoxia (1-3). This is a critical link between glycolysis and the TCA cycle (3). PDH activity is regulated by phosphorylation and dephosphorylation (3). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (3). Hypoxia can directly induce pyruvate dehydrogenase kinase 1 (PDHK1) expression, which results in inactivation of PDH and the TCA cycle and subsequent suppression of metabolism (4).

  1. Harris, R.A. et al. (2002) Adv Enzyme Regul 42, 249-59.
  2. Fries, M. et al. (2003) Biochemistry 42, 6996-7002.
  3. Wigfield, S.M. et al. (2008) Br J Cancer 98, 1975-84.
  4. Kim, J.W. et al. (2006) Cell Metab 3, 177-85.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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