Product Pathways - Metabolism
FAAH1 (C84F1) Rabbit mAb #3829
PhosphoSitePlus® protein, site, and accession data: FAAH
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | M R | Endogenous | 60 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
FAAH1 (C84F1) Rabbit mAb detects endogenous levels of total FAAH1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues from the sequence of human FAAH1 protein.
Western Blotting
Western blot analysis of extracts from rat brain and INS-1 cells using FAAH1 (C84F1) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat cerebellum using FAAH1 (C84F1) Rabbit mAb.
Background
Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling in vivo (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1).
- Blednov, Y.A. et al. (2007) Neuropsychopharmacology 32, 1570-82.
- Cravatt, B.F. et al. (1996) Nature 384, 83-7.
- Cravatt, B.F. et al. (2001) Proc Natl Acad Sci USA 98, 9371-6.
Application References
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Companion Products
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
For Research Use Only. Not For Use In Diagnostic Procedures.
