Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PLD1 Antibody #3832

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 120 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

PLD1 Antibody detects endogenous levels of total PLD1 protein. This antibody does not cross-react with PLD2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human PLD1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells stimulated with PMA for 0, 10, 20 and 30 minutes using PLD1 Antibody.

Background

Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified so far. Both are regulated by protein kinases, small GTPases and Ca2+ (1). PLD1 is phosphorylated at Ser2, Ser561 and Thr147 by PKC (2,3). Phosphorylation at Thr147 and Ser561 regulates PLD1 activity (3).

  1. Exton, J.H. (1999) Biochim. Biophys. Acta. 1439, 121-133.
  2. Kim, Y. et al. (1999) Biochemistry 38, 103244-10351.
  3. Kim, Y. et al. (2000) J. Biol. Chem. 275, 13621-13627.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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