Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

eIF6 (D16E9) XP® Rabbit mAb #3833

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 27 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF6 (D16E9) XP® Rabbit mAb detects endogenous levels of total eIF6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF6.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using eIF6 (D16E9) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using eIF6 (D16E9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3).

  1. Sanvito, F. et al. (1999) J Cell Biol 144, 823-37.
  2. Ceci, M. et al. (2003) Nature 426, 579-84.
  3. Chendrimada, T.P. et al. (2007) Nature 447, 823-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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