Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PLD1 (Ser561) Antibody #3834

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 120 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-PLD1 (Ser561) Antibody detects endogenous levels of PLD1 only when phosphorylated at serine 561. This antibody does not cross-react with phosphorylated PLD2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser561 of human PLD1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 cells, untreated or PMA-stimulated for the indicated times, using Phospho-PLD1 (Ser561) Antibody (upper) or PLD1 Antibody #3832 (lower).

Background

Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified so far. Both are regulated by protein kinases, small GTPases and Ca2+ (1). PLD1 is phosphorylated at Ser2, Ser561 and Thr147 by PKC (2,3). Phosphorylation at Thr147 and Ser561 regulates PLD1 activity (3).

  1. Exton, J.H. (1999) Biochim. Biophys. Acta. 1439, 121-133.
  2. Kim, Y. et al. (1999) Biochemistry 38, 103244-10351.
  3. Kim, Y. et al. (2000) J. Biol. Chem. 275, 13621-13627.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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