Cell Signaling Technology

Product Pathways - Neuroscience

EAAT2 Antibody #3838

Applications Reactivity Sensitivity MW (kDa) Source
W IF-F M R (H) Endogenous 65 Rabbit

Applications Key:  W=Western Blotting  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

EAAT2 Antibody detects endogenous levels of total EAAT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human EAAT2. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using EAAT2 Antibody.

IF-F

IF-F

Confocal immunofluorescent analysis of rat retina using EAAT2 Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3).

  1. Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8.
  2. Tanaka, K. et al. (1997) Science 276, 1699-702.
  3. Casado, M. et al. (1993) J Biol Chem 268, 27313-7.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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