Product Pathways - Neuroscience
EAAT2 Antibody #3838
PhosphoSitePlus® protein, site, and accession data: GLT1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F | M R (H) | Endogenous | 65 | Rabbit |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
EAAT2 Antibody detects endogenous levels of total EAAT2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human EAAT2. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse and rat brain using EAAT2 Antibody.
IF-F
Confocal immunofluorescent analysis of rat retina using EAAT2 Antibody (green). Blue pseudocolor = DRAQ5® #4084(fluorescent DNA dye).
Background
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3).
- Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8.
- Tanaka, K. et al. (1997) Science 276, 1699-702.
- Casado, M. et al. (1993) J Biol Chem 268, 27313-7.
Application References
- Díaz-Amarilla, P. et al. (2011) Proc Natl Acad Sci U S A 108, 18126-31. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
