Product Pathways - Cytoskeletal Signaling
Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Transfected Only | 72 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody detects transfected levels of LIMK1 and LIMK2 only when phosphorylated at threonine 508 or 505.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr508 of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1), transfected with Wild-type LIMK1 (lanes 2 and 3) or with LIMK1 T508A mutant (lanes 4 and 5), using Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (top), LIMK1 Antibody #3842 (middle) or HA-Tag (262K) mAb #2362 (bottom). Cells were either untreated (lanes 1, 2 and 4) or treated with PMA (lanes 3 and 5). (Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.)
Background
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).
- Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
- Maekawa, M. et al. (1999) Science 285, 895-898.
- Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
- Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
- Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
- Arber, S. et al. (1998) Nature 393, 805-809.
- Yang, N. et al. (1998) Nature 393, 809-812.
Application References
- Heredia, L. et al. (2006) J Neurosci 26, 6533-42. This article references the use of Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody in the following applications: IF-IC (In Cells) Western Blotting
- Pandey, D. et al. (2006) Blood 107, 575-83. This article references the use of Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody in the following applications: Western Blotting
- Croft, D.R. and Olson, M.F. (2006) Mol Cell Biol 26, 4612-27. This article references the use of Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody in the following applications: Western Blotting
- Hsieh, S.H. et al. (2006) J Neurosci 26, 1006-15. This article references the use of Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody in the following applications: Western Blotting
- Misra, U.K. et al. (2005) J Immunol 175, 2525-33. This article references the use of Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
