Product Pathways - Cytoskeletal Signaling
LIMK1 Antibody #3842
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 70 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
LIMK1 Antibody detects endogenous levels of total LIMK1 protein independent of phosphorylation. The antibody does not cross-react with LIMK2.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal residues of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HEK293, A431, NIH/3T3, COS and PC12 cells, using LIMK1 Antibody.
Background
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).
- Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
- Maekawa, M. et al. (1999) Science 285, 895-898.
- Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
- Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
- Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
- Arber, S. et al. (1998) Nature 393, 805-809.
- Yang, N. et al. (1998) Nature 393, 809-812.
Application References
- Pandey, D. et al. (2006) Blood 107, 575-83. This article references the use of LIMK1 Antibody in the following applications: IP Western Blotting
- Li, Z. et al. (2006) Mol Cell Biol 26, 4240-56. This article references the use of LIMK1 Antibody in the following applications: Western Blotting
- Gorovoy, M. et al. (2005) J Biol Chem 280, 26533-42. This article references the use of LIMK1 Antibody in the following applications: IF-IC (In Cells) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2601 Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
- 2602 PAK1 Antibody
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- 2607 Phospho-PAK2 (Ser20) Antibody
- 2608 PAK2 Antibody
- 3311 Phospho-Cofilin (Ser3) Antibody
- 3312 Cofilin Antibody
- 3841 Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody
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- 7727 Biotinylated Protein Ladder Detection Pack
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- 3844 LIMK2 Antibody
- 3845 LIMK2 (8C11) Rabbit mAb
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
