Product Pathways - Cytoskeletal Signaling

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LIMK1 Antibody #3842

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk	Endogenous	70	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3842:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

LIMK1 Antibody detects endogenous levels of total LIMK1 protein independent of phosphorylation. The antibody does not cross-react with LIMK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
2. Maekawa, M. et al. (1999) Science 285, 895-898.
3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
6. Arber, S. et al. (1998) Nature 393, 805-809.
7. Yang, N. et al. (1998) Nature 393, 809-812.

Application References

• Pandey, D. et al. (2006) Blood 107, 575-83. Applications: IP Western Blotting
• Li, Z. et al. (2006) Mol Cell Biol 26, 4240-56. Applications: Western Blotting
• Gorovoy, M. et al. (2005) J Biol Chem 280, 26533-42. Applications: IF-IC (In Cells) Western Blotting
• Gamell, C. et al. (2008) J Cell Sci 121, 3960-70. Applications: Western Blotting
• Li, Z. et al. (2006) Mol Cell Biol 26, 4240-56. Applications: Western Blotting
• Park, J.H. et al. (2011) J Biol Chem 286, 23132-41. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2601 Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
• 2602 PAK1 Antibody
• 2605 Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
• 2606 Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
• 2607 Phospho-PAK2 (Ser20) Antibody
• 2608 PAK2 Antibody
• 3311 Phospho-Cofilin (Ser3) Antibody
• 3312 Cofilin Antibody
• 3841 Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 3845 LIMK2 (8C11) Rabbit mAb
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody

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For Research Use Only. Not For Use In Diagnostic Procedures.