Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

LIMK1 Antibody #3842

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIMK1 Antibody detects endogenous levels of total LIMK1 protein independent of phosphorylation. The antibody does not cross-react with LIMK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293, A431, NIH/3T3, COS and PC12 cells, using LIMK1 Antibody.

Background

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

  1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
  2. Maekawa, M. et al. (1999) Science 285, 895-898.
  3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
  4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
  5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
  6. Arber, S. et al. (1998) Nature 393, 805-809.
  7. Yang, N. et al. (1998) Nature 393, 809-812.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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