Product Pathways - Cytoskeletal Signaling
LIMK2 Antibody #3844
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H R Mk (M) | Transfected Only | 70 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
LIMK2 Antibody detects transfected levels of LIMK2. The antibody does not cross-react with LIMK1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to carboxy-terminal residues of human LIMK2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS (lane 1), COS transfected with triple HA-tagged LIMK1 (lane 2) and COS transfected with LIMK2 (lane 3), using LIMK2 Antibody.
Background
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).
- Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
- Maekawa, M. et al. (1999) Science 285, 895-898.
- Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
- Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
- Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
- Arber, S. et al. (1998) Nature 393, 805-809.
- Yang, N. et al. (1998) Nature 393, 809-812.
Application References
- Goyal, P. et al. (2005) J Biol Chem 280, 27569-77. This article references the use of LIMK2 Antibody in the following applications: IP Western Blotting
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Companion Products
- 2601 Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
- 2602 PAK1 Antibody
- 2604 PAK1/2/3 Antibody
- 2605 Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
- 2606 Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
- 2607 Phospho-PAK2 (Ser20) Antibody
- 2608 PAK2 Antibody
- 3311 Phospho-Cofilin (Ser3) Antibody
- 3322 Ras-GRF1 Antibody
- 3841 Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody
- 3842 LIMK1 Antibody
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
