Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

LIMK2 (8C11) Rabbit mAb #3845

Applications Reactivity Sensitivity MW (kDa) Isotype
W F H Mk (M) Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIMK2 (8C11) Rabbit Monoclonal Antibody detects endogenous levels of total LIMK2 protein. The antibody does not cross-react with LIMK1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human LIMK2.

Western Blotting

Western Blotting

Western blot analysis of extracts from Colo201 and Jurkat cells, using LIMK2 (8C11) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using LIMK2 (8C11) Rabbit mAb Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

  1. Okano, I. et al. (1995) J. Biol. Chem. 270, 31321-31330.
  2. Maekawa, M. et al. (1999) Science 285, 895-898.
  3. Edwards, D. C. et al. (1999) Nat. Cell Biol. 1, 253-259.
  4. Ohashi, K. et al. (2000) J. Biol. Chem. 275, 3577-3582.
  5. Sumi, T. et al. (2001) J. Biol. Chem. 276, 670-676.
  6. Arber, S. et al. (1998) Nature 393, 805-809.
  7. Yang, N. et al. (1998) Nature 393, 809-812.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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