Cell Signaling Technology

Product Pathways - Protein Stability

S5a/PSMD4 Antibody #3846

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 49 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

S5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using S5a/PSMD4 Antibody.

Background

S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5).

  1. Deveraux, Q. et al. (1995) J. Biol. Chem. 270, 23726-23729.
  2. Young, P. et al. (1998) J. Biol. Chem. 273, 5461-5467.
  3. Beal, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 861-866.
  4. Fujiwara, K. et al. (2004) J. Biol. Chem. 279, 4760-4767.
  5. Sakata, E. et al. (2003) EMBO Rep. 4, 301-306.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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