Product Pathways - Protein Folding/Stability
E2-25K/Hip2 Antibody #3847
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 25 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
E2-25K/Hip2 Antibody detects endogenous levels of total E2-25K/Hip2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Arg11 of human E2-25K/Hip2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using E2-25K/Hip2 Antibody.
Background
Protein ubiquitinylation requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with E1. The activated ubiquitin is then transferred to a thiol-group of ubiquitin-conjugation enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-conjugating enzyme E3 (1).
E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-5). Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity (6).
- Hershko, A. (1988) J. Biol. Chem. 263, 15237-15240.
- Chen, Z. and Pickart, C.M. (1990) J. Biol. Chem. 265, 21835-21842.
- Song, S. et al. (2003) Mol. Cell 12, 553-563.
- de Pril, R. et al. (2007) Mol. Cell. Neurosci. 34, 10-19.
- Flierman, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11589-11594.
- Pichler, A. et al. (2005) Nat. Struct. Mol. Biol. 12, 264-269.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
