Cell Signaling Technology

Product Pathways - Protein Stability

E2-25K/Hip2 Antibody #3847

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

E2-25K/Hip2 Antibody detects endogenous levels of total E2-25K/Hip2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg11 of human E2-25K/Hip2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using E2-25K/Hip2 Antibody.

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-5). Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity (6).

  1. Hershko, A. (1988) J. Biol. Chem. 263, 15237-15240.
  2. Chen, Z. and Pickart, C.M. (1990) J. Biol. Chem. 265, 21835-21842.
  3. Song, S. et al. (2003) Mol. Cell 12, 553-563.
  4. de Pril, R. et al. (2007) Mol. Cell. Neurosci. 34, 10-19.
  5. Flierman, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11589-11594.
  6. Pichler, A. et al. (2005) Nat. Struct. Mol. Biol. 12, 264-269.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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