Cell Signaling Technology

Product Pathways - Protein Stability

UBE2L3 Antibody #3848

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 17 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

UBE2L3 Antibody detects endogenous levels of total UBE2L3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys145 of human UBE2L3 protein. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using UBE2L3 Antibody.

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

UBCH7 (also called UBE2L3) is an 17 kDa ubiquitin-conjugating enzyme E2 (3). It can interact with different E3 ligase including E6-AP, SRC-1, c-Cbl, parkin and mediates regulation of a wide variety signaling pathways and is associated with diseases such as Parkinson's disease (4-7).

  1. Hershko, A. (1988) J. Biol. Chem. 263, 15237-15240.
  2. Rajendra, R. et al. (2004) J. Biol. Chem. 279, 36440-36444.
  3. Nuber, U. et al. (1996) J. Biol. Chem. 271, 2795-2800.
  4. Verma, S. et al. (2004) Mol. Cell Biol. 24, 8716-8726.
  5. Garside, H. et al. (2006) J. Endocrinol. 190, 621-629.
  6. Shimura, H. et al. (2001) Science 293, 263-269.
  7. Simmons, A. et al. (2005) Immunity 23, 621-634.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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