Product Pathways - Protein Folding/Stability
UBE2L3 Antibody #3848
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 17 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 3848:
- Western Blotting
Specificity / Sensitivity
UBE2L3 Antibody detects endogenous levels of total UBE2L3 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys145 of human UBE2L3 protein. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using UBE2L3 Antibody.
Background
Protein ubiquitinylation requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with E1. The activated ubiquitin is then transferred to a thiol-group of ubiquitin-conjugation enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-conjugating enzyme E3 (1).
UBCH7 (also called UBE2L3) is an 17 kDa ubiquitin-conjugating enzyme E2 (3). It can interact with different E3 ligase including E6-AP, SRC-1, c-Cbl, parkin and mediates regulation of a wide variety signaling pathways and is associated with diseases such as Parkinson's disease (4-7).
- Hershko, A. (1988) J. Biol. Chem. 263, 15237-15240.
- Rajendra, R. et al. (2004) J. Biol. Chem. 279, 36440-36444.
- Nuber, U. et al. (1996) J. Biol. Chem. 271, 2795-2800.
- Verma, S. et al. (2004) Mol. Cell Biol. 24, 8716-8726.
- Garside, H. et al. (2006) J. Endocrinol. 190, 621-629.
- Shimura, H. et al. (2001) Science 293, 263-269.
- Simmons, A. et al. (2005) Immunity 23, 621-634.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
