Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PLCγ Antibody Sampler Kit #3860

Kit Includes Quantity Applications Reactivity MW (kDa) Source
Phospho-PLCγ1 (Tyr783) Antibody # 2821 40 microliters W IP F H M R 155 Rabbit
PLCγ1 Antibody # 2822 40 microliters W IP H M R 155 Rabbit
Phospho-PLCγ2 (Tyr1217) Antibody # 3871 40 microliters W H M (R) 150 Rabbit
PLCγ2 Antibody # 3872 40 microliters W IP H M (R) 150 Rabbit
Phospho-PLCγ2 (Tyr759) Antibody # 3874 40 microliters W IP H M 150 Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074 100 microliters Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat

Specificity / Sensitivity

Each antibody in the PLCγ Antibody Sampler Kit detects endogenous levels of its target protein. The antibodies do not cross react with other PLCs.

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3 cells, untreated or treated with PDGF alone or PDGF and CIP/λ phosphatases, using Phospho-PLCγ1 (Tyr783) Antibody #2821 (upper) or PLCγ1 Antibody #2822 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM, using Phospho-PLCγ2 (Tyr1217) Antibody #3871 (upper), Phospho-PLCγ2 (Tyr 759) Antibody #3874 (middle), or PLCγ2 Antibody #3872 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 (lanes 1, 3, 5) and Ramos (lanes 2, 4, 6) cells, using PLCγ1 Antibody #2822 (lanes 1 and 2), PLCγ2 Antibody (lanes 5 and 6) or both antibodies (lanes 3 and 4). Results show that PLCγ2 Antibody is specific to the 150 kDa PLCγ2 band detected in Ramos cells, while PLCγ1 Antibody #2822 is specifc to the 160 kDa PLCγ1 band detected in both Ramos and NIH/3T3 cells.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues surrounding human PLCγ1 and PLCγ2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε.Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (2).PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B-cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (5,6).

  1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
  2. Margolis, B. et al. (1989) Cell 57, 1101-7.
  3. Kim, H.K. et al. (1991) Cell 65, 435-41.
  4. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.
  5. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.
  6. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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