Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-FRS2-α (Tyr436) Antibody #3861
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M (R) | Endogenous | 80 to 85 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-FRS2-alpha (Tyr436) Antibody detects endogenous levels of FRS2-alpha only when phosphorylated at tyrosine 436. The antibody does not cross-react with unrelated tyrosine-phosphorylated proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr436 of human FRS2-alpha. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells cotransfected with human FRS2-alpha and rat TrkA, untreated or NGF-stimulated, using Phospho-FRS2-alpha (Tyr436) Antibody. (Cell lysates provided by Dr. Susan Meakin, Robarts Research Institute, London, Ontario.)
Background
Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). Activation of the FGFR leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-alpha (SNT1) and FRS2-beta (SNT2) (2), which are phosphorylated by these RTKs. Once they are phosphorylated, they recruit SH2 domain-containing proteins including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6).
- Kouhara, H. et al. (1997) Cell 89, 693-702.
- Ong, S. H. et al. (2000) Mol. Cell. Biol. 20, 979-989.
- Kontaridis, M. I. et al. (2002) Mol. Cell. Biol. 22, 3875-3891.
- Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276, 13049-13056.
- Hadari, Y. R. et al. (1998) Mol. Cell. Biol. 18, 3966-3973.
- Kouhara, M. et al. (1997) Cell 89, 693-702.
Application References
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Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3864 Phospho-FRS2-α (Tyr196) Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
