Cell Signaling Technology

Product Pathways - Adhesion

ILK1 Antibody #3862

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H M R Mk B Endogenous 51 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ILK1 Antibody detects endogenous levels of total ILK1 protein. The antibody may cross-react with ILK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human ILK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, COS, PC12 and C6 cells, using ILK1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with 50 nM non-targeted siRNA (-) or 100 nM ILK1 siRNA (+), using ILK1 Antibody #3862 and Cofilin Antibody #3312. The ILK1 Antibody confirms silencing of ILK1 expression, and the Cofilin Antibody controls for protein loading and ILK1 siRNA specificity.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using ILK1 Antibody.


Background

Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).

  1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.
  2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
  3. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173.
  4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373.
  5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264.
  6. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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