Cell Signaling Technology

Product Pathways - Adhesion

ILK1 Antibody #3862

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P H M R Mk B Endogenous 51 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

ILK1 Antibody detects endogenous levels of total ILK1 protein. The antibody may cross-react with ILK2.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminus of human ILK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, COS, PC12 and C6 cells, using ILK1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using ILK1 Antibody.

Background

Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).

  1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.
  2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
  3. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173.
  4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373.
  5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264.
  6. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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