Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

MOB1 Antibody #3863

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MOB1 Antibody detects endogenous levels of total MOB1 protein (MOB1α and MOB1β).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro15 of human MOB1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MOB1 Antibody.

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1α and MOB1β, that are encoded by two different genes but have 96.3% identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1 and 2 and NDR1 and 2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues by MST1 and/or MST2 (5,10).

  1. Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
  2. Edgar, B.A. (2006) Cell 124, 267-73.
  3. Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
  4. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
  5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
  6. Praskova, M. et al. (2008) Curr Biol 18, 311-21.
  7. Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
  8. Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
  9. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
  10. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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