Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Phospho-FRS2-α (Tyr196) Antibody #3864

Applications Reactivity Sensitivity MW (kDa) Source
W H M (R) Endogenous 85 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-FRS2-alpha (Tyr196) Antibody detects endogenous levels of FRS2-alpha only when phosphorylated at tyrosine 196. The antibody does not cross-react with unrelated tyrosine-phosphorylated proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr96 of human FRS2-alpha. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or stimulated with bFGF and/or treated with calf intestinal phosphatase (CIP) as indicated, using Phospho-FRS2-alpha (Tyr196) Antibody.

Background

Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). Activation of the FGFR leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-alpha (SNT1) and FRS2-beta (SNT2) (2), which are phosphorylated by these RTKs. Once they are phosphorylated, they recruit SH2 domain-containing proteins including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6).

  1. Kouhara, H. et al. (1997) Cell 89, 693-702.
  2. Ong, S. H. et al. (2000) Mol. Cell. Biol. 20, 979-989.
  3. Kontaridis, M. I. et al. (2002) Mol. Cell. Biol. 22, 3875-3891.
  4. Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276, 13049-13056.
  5. Hadari, Y. R. et al. (1998) Mol. Cell. Biol. 18, 3966-3973.
  6. Kouhara, M. et al. (1997) Cell 89, 693-702.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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