Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

BCL2L10 Antibody #3869

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 23 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BCL2L10 Antibody detects endogenous levels of total BCL2L10 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr115 of human BCL2L10. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Westen blot analysis of extracts from various cell lines using BCL2L10 Antibody.

Background

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.

Bcl-2-like 10 (BCL2L10), known as Diva or Boo in mouse (6,7) and Bcl-B in human (8), is a Bcl-2 family member with some unique properties. Expression of the mouse mRNA was detected in multiple embryonic tissues but restricted to adult ovary and testis (6,7); human Bcl-B appears to be more widely expressed (8). BCL2L10 contains BH1, 2, and 4 domains as well as a putative carboxy-terminal transmembrane domain. While some studies report the presence of a pro-apoptotic BH3 domain in BCL2L10, conflicting reports indicate an incomplete or absent BH3 domain (7-9). Similarly, some studies indicate that BCL2L10 induces apoptosis (6,9) while other data implies a role in suppressing cell death (7,8,10). BCL2L10 may function by differentially binding other Bcl-2 family members and through interaction with the apoptosome protein Apaf-1 (6,7). Despite its restricted expression in mice, Diva knockouts were fertile and exhibit no obvious developmental defects (11).

  1. Cory, S. et al. (2003) Oncogene 22, 8590-607.
  2. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.
  3. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.
  4. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.
  5. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
  6. Inohara, N. et al. (1998) J Biol Chem 273, 32479-86.
  7. Song, Q. et al. (1999) EMBO J 18, 167-78.
  8. Ke, N. et al. (2001) J Biol Chem 276, 12481-4.
  9. Lee, R. et al. (2001) Biochim Biophys Acta 1520, 187-94.
  10. Naumann, U. et al. (2001) FEBS Lett 505, 23-6.
  11. Russell, H.R. et al. (2002) Mol Cell Biol 22, 6866-70.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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