Cell Signaling Technology

Product Pathways - DNA Damage

ERCC1 Antibody #3885

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Hm Mk (Dg) Endogenous 39 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ERCC1 Antibody recognizes endogenous levels of total ERCC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ERCC1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and HepG2 cells using ERCC1 Antibody.

IP

IP

Immunoprecipitation of ERCC1 from HeLa cells using ERCC1 Antibody. Western blot was performed using the same antibody. Lane 1 is 5% input.

Background

DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).

  1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
  2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
  3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
  4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
  5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
  6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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