Product Pathways - Glucose Metabolism
Glycogen Synthase (15B1) Rabbit mAb #3886
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R | Endogenous | 84 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Glycogen Synthase (15B1) Rabbit mAb detects endogenous levels of total muscle and liver glycogen synthase protein.
Source / Purification
Glycogen Synthase (15B1) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human glycogen synthase.
Western Blotting
Western blot analysis of extracts from various cell lines, using Glycogen Synthase (15B1) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Glycogen Synthase (15B1) Rabbit mAb.
Background
Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).
- Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
- Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
- Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
- Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3891 Phospho-Glycogen Synthase (Ser641) Antibody
- 3893 Glycogen Synthase Antibody
- 9336 Phospho-GSK-3β (Ser9) Antibody
- 9337 Phospho-GSK-3α (Ser21) (46H12) Mouse mAb
- 5090 Phospho-GSK-3alpha (Ser21) (27E5) Mouse mAb
- 9338 GSK-3alpha Antibody
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
