Product Pathways - Metabolism

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

Glycogen Synthase (15B1) Rabbit mAb #3886

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P	H M R	Endogenous	84	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3886:

IHC / Paraffin, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Glycogen Synthase (15B1) Rabbit mAb detects endogenous levels of total muscle and liver glycogen synthase protein.

Source / Purification

Glycogen Synthase (15B1) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence of human glycogen synthase.

Background

Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).

1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3891 Phospho-Glycogen Synthase (Ser641) Antibody
• 3893 Glycogen Synthase Antibody
• 9336 Phospho-GSK-3β (Ser9) Antibody
• 9337 Phospho-GSK-3α (Ser21) (46H12) Mouse mAb
• 5090 Phospho-GSK-3α (Ser21) (27E5) Mouse mAb
• 9338 GSK-3α Antibody
• 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 5425 Normal Goat Serum

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

---

For Research Use Only. Not For Use In Diagnostic Procedures.