Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

NuMA Antibody #3888

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H Mk Endogenous 238 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NuMA Antibody detects endogenous levels of total NuMA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NuMA.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using NuMA Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using NuMA Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).

  1. Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
  2. Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
  3. Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
  4. Matsuoka, S. et al. (2007) Science 316, 1160-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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