Product Pathways - Lymphocyte Signaling
HS1 (D83A8) XP® Rabbit mAb (Human Specific) #3890
PhosphoSitePlus® protein, site, and accession data: HS1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H | Endogenous | 80 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
HS1 (D83A8) XP® Rabbit mAb (Human Specific) detects endogenous levels of total HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro310 of human HS1.
Western Blotting
Western blot analysis of cell extracts from Raji, Jurkat, and Molt4 using HS1 (D83A8) XP® Rabbit mAb (Human Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HS1 (D83A8) XP® Rabbit mAb (Human Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using HS1 (D83A8) XP® Rabbit mAb (Human Specific).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells (red) and MOLT-4 cells (blue), using HS1 (D83A8) XP® Rabbit mAb (Human Specific).
IF-IC
Confocal immunofluorescent analysis of Jurkat cells (left) versus 293 cells (right) using HS1 (D83A8) XP® Rabbit mAb (Human Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).
- Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
- Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
- Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
- Hata, D. et al. (1994) Immunol Lett 40, 65-71.
- Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
- Gomez, T.S. et al. (2006) Immunity 24, 741-52.
- Kahner, B.N. et al. (2007) Blood 110, 2449-56.
Application References
- Cavnar, P.J. et al. (2012) J Biol Chem 287, 25466-77. Applications: IF-IC (In Cells) Western Blotting
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 4503 HS1 Antibody (Human Specific)
- 4507 Phospho-HS1 (Tyr397) Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
