Cell Signaling Technology

Product Pathways - Metabolism

Glycogen Synthase Antibody #3893

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H M R Endogenous 84 kDa Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Glycogen Synthase Antibody detects endogenous levels of total muscle glycogen synthase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, C6 and 293 cells, using Glycogen Synthase Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated 293 cells, using Glycogen Synthase Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).

  1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
  2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
  3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
  4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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