Product Pathways - Glucose Metabolism
Glycogen Synthase Antibody #3893
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP F | H M R | Endogenous | 84 kDa | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Glycogen Synthase Antibody detects endogenous levels of total muscle glycogen synthase protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3, C6 and 293 cells, using Glycogen Synthase Antibody.
Flow Cytometry
Flow cytometric analysis of untreated 293 cells, using Glycogen Synthase Antibody (blue) compared to a nonspecific negative control antibody (red).
Background
Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).
- Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
- Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
- Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
- Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
