Product Pathways - Metabolism
Glycogen Synthase Antibody #3893
PhosphoSitePlus® protein, site, and accession data: GYS1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP F | H M R | Endogenous | 84 kDa | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3893:
- Flow, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Glycogen Synthase Antibody detects endogenous levels of total muscle glycogen synthase protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3, C6 and 293 cells, using Glycogen Synthase Antibody.
Flow Cytometry
Flow cytometric analysis of untreated 293 cells, using Glycogen Synthase Antibody (blue) compared to a nonspecific negative control antibody (red).
Background
Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).
- Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
- Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
- Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
- Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
Application References
- Fuchs, G. et al. (2011) J Mol Biol 410, 118-30. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
